면역계 질환을 가진 실험동물에서의 단기간 고중력 노출에 의한 영향 평가
Effect of the Short-Term High G-force in Mice with Immunologic Disorders
인하대학교 의과대학 이비인후과학교실
Department of Otorhinolaryngology, Head and Neck Surgery, Inha University College of Medicine, Incheon, Korea
Received: May 10, 2014; Accepted: May 20, 2014
Korean J Aerosp Environ Med 2014; 24(2): 21-27
Published August 31, 2014
Copyright © Aerospace Medical Association of Korea.
We aimed to study about the effect of acute hyper-gravity (HG) on the allergic immune response in a murine model of allergic asthma. Thirty-two BALB/c mice were used. In Group A (control group, n=8), mice were sensitized and challenged with saline. Group B (HG control group, n=8) were exposed to HG (10 Gz, 1 hour) after intraperitoneal and intranasal saline challenge. Group C (asthma group, n=8) received intraperitoneal and intranasal ovalbumin (OVA) challenge. Group D (HG asthma group, n=8) were exposed to HG after intraperitoneal and intranasal OVA challenge. We evaluated serum total and OVA-specific IgE; serum titers of cytokines; and histopathologic examination of lung. As a result, titers of Serum total and OVA-specific IgE were not significantly different between groups. Compared to Group C, mice in Group D showed significant increase of Th2 cytokines (IL-4, IL-13), cytokines involved in eosinophilia (IL-3, IL-5, GM-CSF) and those involved in cell-medicated immunity (IFN-γ). In histopathologic examination, lungs of Group D showed significantly more infiltration of inflammatory cells compared to Group C. However, these differences were not so significant between Groups A and B. In conclusion, acute HG could exacerbate allergic asthma in experimental animals.
Hypergravity,Asthma,Allergy and immunology,Cytokines